Screening peptide-ore interactions with a phage display library for bioflotation application

CIM Journal, Vol. 7, No. 3, 2016

K. Tremblay-Bouliane, D. Tremblay, S. Moineau, B. Gaillet, and A. Garnier
Université Laval, Québec City, Quebec, Canada

C. Olsen
COREM, Québec City, Quebec, Canada

http://dx.doi.org/10.15834/cimj.2016.20
Abstract A heptapeptide library displayed on M13 bacteriophages (phages) was screened to identify peptide sequences able to selectively bind to gold, chalcopyrite, sphalerite, pyrite, and silica ores, and to evaluate phage potential as bioflotation reagents. Many peptide sequences were isolated through biopanning and differed from known sequences. Adsorption isotherms were determined and a receptor/ligand interaction model was identified and used to compare affinity of peptide sequences toward ores. Some phage sequences have slightly lower affinity to sphalerite than other sulphides. Adsorption was higher on sulphides than on silica. Induction time measurements suggested filamentous M13 phages lower hydrophobicity of the ore surface.
Keywords: Adsorption isotherm, Biosourced, Collector, Depressant, Froth flotation, Hydrophobicity, Induction time, Peptides, Phage display, Screening
Résumé Une librairie d’heptapeptides présentés sur des bactériophages M13 (phages) a été criblée afin d’identifier les séquences peptidiques affinitaires à des minerais d’or, de chalcopyrite, de sphalérite, de pyrite et de silice et d’évaluer le potentiel de ces phages en tant que de bio-réactifs de flottation. De nombreuses séquences peptidiques ont été isolées par bio-criblage et différenciées d’autres séquences connues. Des isothermes d’adsorption ont été déterminées et un modèle d’interaction récepteur–ligand a été identifié et utilisé pour comparer l’affinité des séquences peptidiques par rapport aux minerais. Certaines séquences de phages possèdent une affinité légèrement inférieure pour la sphalérite comparativement aux autres sulfures. L’adsorption sur les sulfures était plus élevée que sur la silice. Des mesures de temps d’induction suggèrent que les phages filamenteux M13 diminuent l’hydrophobicité de la surface des minerais.
Mots-Clé Collecteur, criblage, dépresseur, expression des phages, flottation par écume,hydrophobicité, isotherme d’adsorption, peptides, source biologique, temps d’induction
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